Flint Animal Cancer Center

Pathology/Immuno-Pathology

The Pathology/Immuno-Pathology laboratory is dedicated to the development and use of biomarker assays in cancer of companion and laboratory animals. New automated staining and quantitation technology, study design and planning, as well as veterinary pathology expertise are available. 

Dr. Regan’s research focus includes the interplay between the immune system and tumor stroma, and how these two compartments of the tumor microenvironment respond and mediate resistance to anti-cancer therapy. 

Dr. MacNeill’s research interests include isolation and characterization of canine and feline tumor cells and the study of poxviruses as anticancer agents.

Support and Services

  • Project design and consultation

  • Interpretation of clinical pathological tests (complete blood count, serum chemistry analysis, urinalysis, etc.)

  • Necropsy, dissection and trimming

  • Sample preparation and submission including decalcification

  • Histopathological and cytological evaluation (includes grading, scoring, etc.)

  • Immunohistochemistry (IHC), Immunocytochemistry (ICC), and Immunofluorescence (IF)

  • IHC, ICC and IF protocol development

  • Western Blot/Dot Blot with quantification

  • Droplet Digital PCR protocol development

  • Image collection, analysis and digital pathology

  • Data collection and interpretation

Equipment

  • Dako Automated Stainer for routine tissue and IHC.

  • 1D and 2D gel electrophoresis

  • Zeiss Automated microscope with image analysis and epifluorescence

  • Leica inverted fluorescent microscope with imaging capabilities

  • Bio-Rad droplet digital PCR reader

  • Spectrophotometer/fluorometer/luminometer microplate reader

  • Ultracentrifuge