AboutThe Cancer Biology & Experimental Therapeutics laboratory has developed and maintains approximately 30 canine and 4 feline cell lines for use in screening and identification of novel products. Cell lines have been validated and are in the process of undergoing expression profiling, DNA and miRNA sequencing. Common molecular assays and in vivo xenograft and bioluminescence studies are available.
Dr. Thamm received his Bachelor’s and V.M.D. degrees from the University of Pennsylvania. Following graduation, he completed an oncology residency at the University of Wisconsin, and was a researcher there for five additional years before joining the CSU faculty in 2004. He is the Barbara Cox Anthony Professor of Oncology at the Flint Animal Cancer Center. He is also a member of the Developmental Therapeutics Section of the University of Colorado Comprehensive Cancer Center and the Cell and Molecular Biology Graduate Program at Colorado State University.
Dr. Thamm’s research centers on the characterization of important therapeutic targets in human oncology in spontaneous tumors of animal species sharing the environment with man, e.g. dogs and cats. Through the implementation of pharmacokinetically and pharmacodynamically intensive animal clinical trials, his laboratory seeks to provide meaningful translational information to inform and develop novel therapies with potential to impact the treatment of human and animal cancers. His current work focuses on tyrosine and serine/threonine kinase signaling, validation of biomarkers for novel therapeutics, and targeted therapy to enhance chemosensitivity.
In vitro and murine preclinical assays for drug activity and target inhibition in canine and feline cells
30 validated canine and 4 feline tumor cell lines representing a variety of common cancer types
Cell proliferation, apoptosis, migration, invasion, cell-cycle
Cytokine and growth factor ELISA
Western analysis, immunoprecipitation, real-time PCR
Immunohistochemistry, immunocytochemistry, immunofluorescence
Mouse xenograft experiments
Bio-Tek Synergy HT Multi-Mode Microplate Reader
Stratagene MX3000P real-time PCR workstation
Xenogen IVISTM luciferase and GFP in vivo imaging system
- Bio-Rad ChemiDoc XRS Gel Imaging System